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Mem. Inst. Oswaldo Cruz ; 98(4): 461-463, June 2003. ilus, tab
Article in English | LILACS | ID: lil-344235

ABSTRACT

We analyzed, by env and gag heteroduplex mobility assay, 149 human immunodeficiency virus (HIV-1) positive samples collected in Ceará during the year 2000. The prevalence of subtype B was 81.2 percent and the prevalence of subtype F and B/F recombinants were both 2.7 percent. Eight (5.4 percent) and 12 (8 percent) out of 149 samples showed indeterminate results in the env and gag analysis respectively. By FokI restriction fragment length polymorphism, 34 percent of the subtype B samples were identified as the typical Brazilian subtype B.In the present study, we identified HIV-1 subtype F and B/F in Ceará for the first time. Our results contribute to the understanding of HIV in Brazil, and may prove useful for the development of vaccine candidates


Subject(s)
Child , Adolescent , Adult , Humans , Male , Female , Middle Aged , DNA, Viral , Genes, env , Genes, gag , HIV Infections , HIV-1 , Brazil , Genetic Variation , Heteroduplex Analysis , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Prevalence
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